Analysis of soil and roots, tubers, leaves and seeds

Soil samples are processed using the centrifugal flotation method in sucrose solution (Jenkins, 1964), and root samples are processed using the Coolen & D'Herde method (1972).

Procedure for collecting samples

- Collection season
Samples should preferably be collected in the cycle prior to a new planting and at times when the moisture level is adequate for plant development, that is, when the soil is neither waterlogged nor too dry.

- How to collect and package samples
Collecting samples for nematological analysis requires a specific procedure to accurately assess the existing population in the field or nursery. Dig the soil around each plant to be sampled to a depth of 0 to 30 cm, collecting the soil and fine roots (rootlets), taking care not to break them. Uniformize the soil obtained at the sampling points and remove approximately 500g. Similarly, uniformize the roots and remove approximately 100 grams. Roots and soil should be packaged together or separately in plastic bags, which should be sealed, labeled, and kept in a cool place until sent to the laboratory.